Application

Research Sub CategoryLive Cell Dye

Live cell fluorescent imaging

Live cell imaging dye for cellular nitric oxide (NO) localized to mitochondria and lysosome, used as an indicator of host defense, homeostasis and developmental functions.

Research CategoryCell Imaging

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nitric oxide (No) is a reactive nitrogen species which is produced by nitric oxide synthase (NOS). NO is involved in many physiological processes including regulation of blood pressure, homeostasis, activation of immune system, neural communication, contraction of both smooth muscle and vascular tissue. Therefore, detection and quantification of NO is critical to understanding health and disease.The BioTracker﹤TMSYMBOL>﹤/TMSYMBOL> SiRNO-Nitric oxide dye is a live cell near-infrared fluorescent imaging probe for detecting nitric oxide (NO). The probe targets NO specifically in the mitochondria and lysosome of living cells. The probe shows no cross reactivity to a variety of biomolecules and other reactive species including: ascorbic acid (AA), dehydroascorbic acid (DHA), methylglyoxal (MGO), GSH, Cys, Hcy, H2O2, ClO−, -OH, O2−, NO2−, and ONOO−. The SiRNO dye is pH insensitive in the range of 5.5 - 8.0, showing that it is suitable for physiological pH range. The SiRNO dye has been successfully used to detect NO in live cells, animal tissue and in vivo experiments.Spectral PropertiesAbsorbance: 633nmEmission: 650-750nm

Physical form

Lyophilized

Quality

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 758.34 g/mol

Storage and Stability

Store BioTracker SiRNO-Nitric Oxide Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.